Abstract: The development of modified sorbents combining the adsorption properties of the initial porous material and the biological activity of the modifiers is a сurrent issue of sorption therapy. The purpose of the study was to develop a method for modifying carbon sorbents with hydroxyacids and to conduct a comparative assessment of their effectiveness upon poisoning animals with ivermectin. A granular carbon enterosorbent with a specific surface area of 300-400 m2/g was the matrix for the preparation of biospecific sorbents, glycolic acid (50 wt.%, manufactured by Merk Schuchardt OHG, Germany) and lactic acid (80 wt.%, produced by MOSREACTIVE, Russia) and a copolymer thereof were used as modifiers. To study the morphology and surface topography of the obtained samples, scanning electron microscopy (JSM 6460LV, JEOL Ltd, Japan) was used. The therapeutic efficacy of the modified sorbents was measured in 5 groups (10 animals each) of outbred adult white rats weighing 180-200 g. During 5 days animals of different groups received one of the modified sorbents in doses of 0.3 g/kg upon acute intoxication with Baymec® (1 % solution of ivermectin, Bayer Animal Health GmbH, Germany; manufactured by Federal Centre for Animal Health, Russia). The controls were intact and intoxicated animals. At the end of the experiment, blood was taken from all animals for biochemical studies, as well as histological examination of organs and tissues was performed post mortem. Electron microscopic examination showed that upon modification, the size and shape of the granules of the initial sorbent are preserved, but surface topography and morphology change. The results of in vivo experiments showed varying degrees of efficiency of enterosorption in rats when modified sorbents were applied to the animals intoxicated with ivermectin-containing Baymec® preparation. Creatinine varied from 40.86±0.66 μmol/l (p < 0.0001) for the sorbent with lactic acid to 82.32±2.74 μmol/l (p = 0.4698) for the sorbent with glycolic acid; in intoxicated animals, the creatinine level reached 87.00±5.52 μmol/l, p < 0.0001). Intoxication was accompanied by an increase in the total protein content in blood of rats (73.08±0.96 g/l, р = 0.0001), mainly due to globulins. At the same time, the concentration of urea decreased (2.44±0.05 mmol/l, р = 0.0001) but the content of total bilirubin increased (1.80±0.07 μmol/l, р = 0.0379), while the level of creatinine increased 2.3 times compared to intact animals. Post-mortem analysis indicated the polytropic nature of the drug. In animals, acute congestive hyperemia of the internal organs and the brain was found. Under the epicardium, petechial hemorrhages were noticeable. There were also marked the heart dilatation, granular dystrophy of cardiomyocytes, protein-fatty degeneration of the liver, emphysematous changes in the lungs, congestive hyperemia and hemorrhages in the kidneys. In the gastrointestinal tract signs of acute catarrh were prevailing. The most distinct corrective effect of enterosorption was noted when using a sorbent modified with lactic acid oligomer. The research results confirm the prospects for the development of bifunctional sorbents as well as expand the possibilities of sorption therapy.

Cite: Dorozhkin V.I. , Fedorov Y.N. , Gerunova L.K. , P’yanova L.G. , Gerunov T.V. , Delyagina M.S. , Tarasenko A.A.
Therapeutic efficiency of sorbents modified by hydroxic acids during animal experimental poisoning with ivermectin
Agricultural Вiology. 2020. V.55. N2. P.394-405. DOI: 10.15389/agrobiology.2020.2.394eng Scopus OpenAlex

Original: Дорожкин В.И. , Федеров Ю.Н. , Герунова Л.К. , Пьянова Л.Г. , Герунов Т.В. , Делягина М.С. , Тарасенко А.А.
Терапевтическая эффективность модифицированных гидроксикислотами сорбентов при травлении животных ивермектином в условиях эксперимента
Сельскохозяйственная биология. 2020. Т.55. №2. С.394-405. DOI: 10.15389/agrobiology.2020.2.394rus РИНЦ OpenAlex

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Dates:

Submitted:	Dec 20, 2019
Published print:	May 1, 2020

Identifiers:

Scopus:	2-s2.0-85088298304
OpenAlex:	W3021925778

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